Journal: Cell metabolism

Article Title: Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue

doi: 10.1016/j.cmet.2022.02.016

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: CD86 Antibody, anti-mouse, FITC, Miltenyi Biotec , Miltenyi Biotec , Cat# 130–123-672; RRID:AB_2889633.

Techniques: Immunofluorescence, Recombinant, Cytometry, Purification, shRNA, Cell Culture, Lysis, XF Assay, Staining, Isolation, Transfection, Live Cell Imaging, Mouse Assay, Software, Expressing

Journal: Chemical Science

Article Title: Bimodal accurate H 2 O 2 regulation to equalize tumor-associated macrophage repolarization and immunogenic tumor cell death elicitation †

doi: 10.1039/d4sc06305h

Figure Lengend Snippet: In vivo antitumor efficacy of ZnO 2 -ATM: inhibition of metastatic tumor growth and immune activation. (A) Primary tumor growth curves of various treatment groups. (B) Images of excised primary tumors after sacrificing mice at 14 days post-injection. (C) H&E-, TUNEL- and CRT-stained immunofluorescence of primary tumor sections at 1 day post-injection after ZnO 2 -ATM treatment. (D) Population changes of M1 (CD80 high CD206 low ) macrophages in primary tumors after various treatments at 3 days post-injection. (E) Population changes of maturing DCs (CD80 high CD86 high ) in spleens after various treatments at 3 days post-injection (gating on CD11c + ). (F) Distant tumor growth curves of various treatment groups. (G) Number of metastatic nodules in lungs after various treatments with the inset graph showing the corresponding lungs with metastatic nodules. (H) H&E-stained lung sections at 44 days post-injection. Red arrows indicate metastases.

Article Snippet: CRT antibodies, HMGB-1 antibodies, APC-conjugated CRT secondary antibodies and Alexa Fluor 594-conjugated HMGB secondary antibodies were purchased from Univ Biotech Co., Ltd. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was obtained from Biofrox Co., Ltd. A TUNEL apoptosis detection kit (C1086) was purchased from Beyotime Biotechnology Co., Ltd. A TNF-α ELISA kit, an IL-6 ELISA kit, APC-conjugated anti-mouse CD80 (CD80-APC), FITC-conjugated anti-mouse CD11b (CD11b-FITC), FITC-conjugated anti-mouse CD11c (CD11c-FITC), PE-conjugated anti-mouse CD206 (CD206-PE), APC-conjugated anti-mouse CD3 (cat. 100236), PE-conjugated anti-mouse CD4 (CD4-PE), FITC-conjugated anti-mouse CD8a (CD8a-FITC), PE-conjugated anti-mouse CD86 (CD86-PE), and PE-conjugated anti-mouse CD44 (CD44-PE) were obtained from Multi-Science Co., Ltd. Annexin V-FITC/propidium iodide (PI) and 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) were purchased from Yeasen Co., Ltd. Deionized water was generated using a Millipore Milli-Q system (Billerica, MA, USA).

Techniques: In Vivo, Inhibition, Activation Assay, Injection, TUNEL Assay, Staining, Immunofluorescence

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Anticancer Activity of Liquid Treated with Microwave Plasma-Generated Gas through Macrophage Activation

doi: 10.1155/2020/2946820

Figure Lengend Snippet: Depolarization of IL-4-pretreated Raw 264.7 macrophages at 24 h elapsed from PGNO-media treatment in various dilution ratios: (a, b) transcriptional level changes of macrophage M1 polarization-related genes, (a) including iNOS, IL6, and TNF- α , and (b) M2 polarization-related genes, including ARG, IL10, TGF- β , CCL17, EGF, and MMP9. (c) Protein level changes of iNOS. All tests were repeated three times. ( ∗ p < 0.05; † p < 0.01). (d) Flow cytometry analysis of iNOS, CD86, and CD163 proteins.

Article Snippet: LPS (L4391, Sigma) was used with a concentration of 10 ng/mL, SNAP (S-Nitroso-N-Acetyl-D,L-Penicillamine; Sigmal) was used with a concentration of 50 μ M, and cPTIO (2-(4-carboxyphenyl)-4,5-dihydro-4,4,5,5-tetramethyl-1H-imidazolyl-1-oxy-3-oxide, monopotassium salt; Cayman) was used with a concentration of 50 μ M. For staining with antibodies, anti-iNOS-PE (12-5920-80, eBioscience), anti-CD163-PE (bs-2527R-PE, Bioss), and anti-CD86-FITC (bs-1035R-FITC, Bioss) were used.

Techniques: Flow Cytometry

Journal: Molecules

Article Title: The Main Structural Unit Elucidation and Immunomodulatory Activity In Vitro of a Selenium-Enriched Polysaccharide Produced by Pleurotus ostreatus

doi: 10.3390/molecules27082591

Figure Lengend Snippet: Effects of Se-POP-21 on the expression of CD80 ( a ) and CD86 ( b ) in RAW264.7 cells, as measured by flow cytometry. The data are expressed as mean ± SD; ** p < 0.01 compared with the control group.

Article Snippet: CD80 and CD86 antibodies (FITC) were purchased from Sino Biological Inc. (Beijing, China).

Techniques: Expressing, Flow Cytometry